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anti il 1b  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology anti il 1b
    Anti Il 1b, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1563 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/il-1b+antibody/IL-1%CE%B2+Antibody/pm41581720-85-25-27
    Average 96 stars, based on 1563 article reviews
    anti il 1b - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Microglia regulate cortical remyelination via TNFR1-dependent phenotypic polarization.
    Article Snippet: Slides were incubated overnight with the primary antibody at 4 C. Primary antibodies for human multiplex immunofluorescent labeling included caspase1 (1:1500, Proteintech #22915-1-AP), Iba1 (1:5000,Wako #019–19741), IL-18 (1:1000 Abcam #ab191152), IL-1b (1:500, Santa Cruz Biotechnology #sc1250).

    Article Title: Microglia regulate cortical remyelination via TNFR1-dependent phenotypic polarization.
    Article Snippet: Primary antibodies for human immunohistopathology included caspase1 (1:1000, Proteintech #22915-1-AP), Iba1 (1:3000, FUJIFILM Wako #019–19741), IL-18 (1:150, Abcam #ab191152), IL-1b (1:100, Santa Cruz Biotechnology #sc1250).

    Article Title: Antiallergic effect of gami-hyunggyeyeongyotang on ovalbumin-induced allergic rhinitis in mouse and human mast cells.
    Article Snippet: The tissue was then washed three times, and treated with primary antibody overnight at 4 C. Caspase-1 and IL-1b (Santa Cruz Biotechnology) were used as the primary antibodies in a solution with a mixture of bovine serum albumin (GenDepot, Katy, TX, USA) and Triton X100 with PBS.

    Article Title: IDO-Expressing Fibroblasts Protect Islet Beta Cells From Immunological Attack and Reverse Hyperglycemia in Non-Obese Diabetic Mice.
    Article Snippet: Indoleamine 2,3-dioxygenase (IDO) induces immunological tolerance in physiological and pathological conditions.. Therefore, we used dermal fibroblasts with stable IDO expression as a cell therapy to: (i) Investigate the factors determining the efficacy of this cell therapy for autoimmune diabetes in non-obese diabetic (NOD) mice; (ii) Scrutinize the potential immunological mechanisms.. Newly diabetic NOD mice were randomly injected with either 10 10 (10M) or 15 10 (15M) IDO-expressing dermal fibroblasts.

    Article Title: The decoy oligodeoxynucleotide against HIF-1α and STAT5 ameliorates atopic dermatitis-like mouse model
    Article Snippet: The primary antibodies used in this study were as follows: anti-Bax, Bcl-2, COX-2, HIF-1a, IL-4, IL-1b, iNOS, VEGF (Santa Cruz Biotechnology), anti-TNF-a, tryptase, TSLP, p-STAT5, t-STAT5 (Abcam), anti-Bcl-XL, cleaved caspase-3, cyclin D3, cytochrome c, GAPDH (Cell Signaling, Beverly, MA), and anti-filaggrin (Enzo Life Sciences).

    Article Title: Targeting the NLRP3 Inflammasome With Inhibitor MCC950 Prevents Aortic Aneurysms and Dissections in Mice
    Article Snippet: The membranes were blocked for 1 hour in blocking solution comprising Tris-buffered saline containing 5% nonfat dried milk and 0.5% Tween 20 and were incubated overnight with primary antibodies against the following proteins: NLRP3 (AdipoGen Life Sciences), caspase-1 (Santa Cruz Biotechnology), IL-1b (Santa Cruz), and MMP-9 (Santa Cruz).

    Article Title: Mogrol attenuates lipopolysaccharide (LPS)-induced memory impairment and neuroinflammatory responses in mice.
    Article Snippet: This study aimed to evaluate whether mogrol, a main bioactive ingredient of Siraitia grosvenorii, could attenuate LPS-induced memory impairment in mice.. The behavioral tests and immunohistochemical analysis and Western blot were performed.. The present results showed that oral administration of mogrol (20, 40, 80mg/kg) significantly improved LPS-induced memory impairment in mice.

    Article Title: A transepithelial pathway delivers succinate to macrophages, thus perpetuating their pro-inflammatory metabolic state.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIERR Antibodies SUCNR1/GPR91 Novusbio Cat#NBP1-00861 IL-1b Santa Cruz Biotechnology Cat#H-153 donkey anti-goat IgG-HRP Santa Cruz Biotechnology Cat#Sc-2020 CD206 R&D Systems Cat#AF2535 Goat anti-Rabbit IgG (H+L) CrossAdsorbed Secondary Antibody, HRP Thermo Fisher Cat#G21234 b-Actin Sigma-Aldrich Cat#A3854 F4/80 Biogems Cat#02922-80; Clone BM8.1 CD16/CD32 Biogems Cat#08212; Clone 2.4G2 CD11b Biolegend Cat#101215; Clone M1/70 CD80 Biolegend Cat#104725; Clone 16-10A1 SLC26A6 Santa Cruz Cat#sc-515230 SUCNR1 Abbexa Cat#abx318542 Goat anti-Mouse IgG (H+L) CrossAdsorbed Secondary Antibody, Alexa Fluor 488 Invitrogen Cat#A-11001 Goat Anti-Rabbit IgG Antibody (H+L), Biotinylated, R.T.U VECTOR laboratories Cat#BP-9100-50 Biological samples Human fecal samples, serum, gut tissues Severance Hospital N/A Chemicals, peptides, and recombinant proteins cOmplete, EDTA-free Protease Inhibitor Cocktail Roche Cat#11873580001 Pierce Lane Marker Reducing Sample Buffer Thermo Fisher Scientific Cat#39000 Citric acid Sigma-Aldrich Cat#C0759 N-METHYL-D-GLUCAMINE Sigma-Aldrich Cat#M2004 Sodium succinate Sigma-Aldrich Cat#14160 Succinic acid Sigma-Aldrich Cat#S3674 Dextran sulfate sodium (DSS) MP Biomedicals Cat#160110 Chlorophenylalanine Sigma-Aldrich Cat#C6506 2,4,6-trinitrobenzene sulfonic acid (TNBS) Thermo Fisher Scientific Cat#28997 Murine M-CSF Peprotech Cat#315-02 IL-13 Peprotech Cat#210-13 IL-4 Peprotech Cat#214-14 IFNg Peprotech Cat#315-05 thioglycolate Hylabs Cat#TT137 70-mm strainer FLACON Cat#FAL352350 LPS Sigma-Aldrich Cat#L2880 14C-Succinic acid ViTrax Inc Cat#VC 195 3H-Citric acid ViTrax Inc Cat#VT 295 SLC13A5 inhibitor BI01383298 TOCRIS Bioscience CAS#2227549-00-8 TRIzol Thermo Fisher Scientific Cat#15596018 SYBR Green master mix Thermo Fisher Scientific Cat#4368708 Horse serum Vector Cat#S-2000 (Continued on next page) e1 Cell Reports 36, 109521, August 10, 2021

    Multiplex Assay:

    Article Title: Microglia regulate cortical remyelination via TNFR1-dependent phenotypic polarization.
    Article Snippet: Slides were incubated overnight with the primary antibody at 4 C. Primary antibodies for human multiplex immunofluorescent labeling included caspase1 (1:1500, Proteintech #22915-1-AP), Iba1 (1:5000,Wako #019–19741), IL-18 (1:1000 Abcam #ab191152), IL-1b (1:500, Santa Cruz Biotechnology #sc1250).

    Article Title: Microglia regulate cortical remyelination via TNFR1-dependent phenotypic polarization.
    Article Snippet: Primary antibodies for human immunohistopathology included caspase1 (1:1000, Proteintech #22915-1-AP), Iba1 (1:3000, FUJIFILM Wako #019–19741), IL-18 (1:150, Abcam #ab191152), IL-1b (1:100, Santa Cruz Biotechnology #sc1250).

    Article Title: Antiallergic effect of gami-hyunggyeyeongyotang on ovalbumin-induced allergic rhinitis in mouse and human mast cells.
    Article Snippet: The tissue was then washed three times, and treated with primary antibody overnight at 4 C. Caspase-1 and IL-1b (Santa Cruz Biotechnology) were used as the primary antibodies in a solution with a mixture of bovine serum albumin (GenDepot, Katy, TX, USA) and Triton X100 with PBS.

    Article Title: IDO-Expressing Fibroblasts Protect Islet Beta Cells From Immunological Attack and Reverse Hyperglycemia in Non-Obese Diabetic Mice.
    Article Snippet: Indoleamine 2,3-dioxygenase (IDO) induces immunological tolerance in physiological and pathological conditions.. Therefore, we used dermal fibroblasts with stable IDO expression as a cell therapy to: (i) Investigate the factors determining the efficacy of this cell therapy for autoimmune diabetes in non-obese diabetic (NOD) mice; (ii) Scrutinize the potential immunological mechanisms.. Newly diabetic NOD mice were randomly injected with either 10 10 (10M) or 15 10 (15M) IDO-expressing dermal fibroblasts.

    Article Title: The decoy oligodeoxynucleotide against HIF-1α and STAT5 ameliorates atopic dermatitis-like mouse model
    Article Snippet: The primary antibodies used in this study were as follows: anti-Bax, Bcl-2, COX-2, HIF-1a, IL-4, IL-1b, iNOS, VEGF (Santa Cruz Biotechnology), anti-TNF-a, tryptase, TSLP, p-STAT5, t-STAT5 (Abcam), anti-Bcl-XL, cleaved caspase-3, cyclin D3, cytochrome c, GAPDH (Cell Signaling, Beverly, MA), and anti-filaggrin (Enzo Life Sciences).

    Article Title: Targeting the NLRP3 Inflammasome With Inhibitor MCC950 Prevents Aortic Aneurysms and Dissections in Mice
    Article Snippet: The membranes were blocked for 1 hour in blocking solution comprising Tris-buffered saline containing 5% nonfat dried milk and 0.5% Tween 20 and were incubated overnight with primary antibodies against the following proteins: NLRP3 (AdipoGen Life Sciences), caspase-1 (Santa Cruz Biotechnology), IL-1b (Santa Cruz), and MMP-9 (Santa Cruz).

    Article Title: Mogrol attenuates lipopolysaccharide (LPS)-induced memory impairment and neuroinflammatory responses in mice.
    Article Snippet: This study aimed to evaluate whether mogrol, a main bioactive ingredient of Siraitia grosvenorii, could attenuate LPS-induced memory impairment in mice.. The behavioral tests and immunohistochemical analysis and Western blot were performed.. The present results showed that oral administration of mogrol (20, 40, 80mg/kg) significantly improved LPS-induced memory impairment in mice.

    Article Title: A transepithelial pathway delivers succinate to macrophages, thus perpetuating their pro-inflammatory metabolic state.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIERR Antibodies SUCNR1/GPR91 Novusbio Cat#NBP1-00861 IL-1b Santa Cruz Biotechnology Cat#H-153 donkey anti-goat IgG-HRP Santa Cruz Biotechnology Cat#Sc-2020 CD206 R&D Systems Cat#AF2535 Goat anti-Rabbit IgG (H+L) CrossAdsorbed Secondary Antibody, HRP Thermo Fisher Cat#G21234 b-Actin Sigma-Aldrich Cat#A3854 F4/80 Biogems Cat#02922-80; Clone BM8.1 CD16/CD32 Biogems Cat#08212; Clone 2.4G2 CD11b Biolegend Cat#101215; Clone M1/70 CD80 Biolegend Cat#104725; Clone 16-10A1 SLC26A6 Santa Cruz Cat#sc-515230 SUCNR1 Abbexa Cat#abx318542 Goat anti-Mouse IgG (H+L) CrossAdsorbed Secondary Antibody, Alexa Fluor 488 Invitrogen Cat#A-11001 Goat Anti-Rabbit IgG Antibody (H+L), Biotinylated, R.T.U VECTOR laboratories Cat#BP-9100-50 Biological samples Human fecal samples, serum, gut tissues Severance Hospital N/A Chemicals, peptides, and recombinant proteins cOmplete, EDTA-free Protease Inhibitor Cocktail Roche Cat#11873580001 Pierce Lane Marker Reducing Sample Buffer Thermo Fisher Scientific Cat#39000 Citric acid Sigma-Aldrich Cat#C0759 N-METHYL-D-GLUCAMINE Sigma-Aldrich Cat#M2004 Sodium succinate Sigma-Aldrich Cat#14160 Succinic acid Sigma-Aldrich Cat#S3674 Dextran sulfate sodium (DSS) MP Biomedicals Cat#160110 Chlorophenylalanine Sigma-Aldrich Cat#C6506 2,4,6-trinitrobenzene sulfonic acid (TNBS) Thermo Fisher Scientific Cat#28997 Murine M-CSF Peprotech Cat#315-02 IL-13 Peprotech Cat#210-13 IL-4 Peprotech Cat#214-14 IFNg Peprotech Cat#315-05 thioglycolate Hylabs Cat#TT137 70-mm strainer FLACON Cat#FAL352350 LPS Sigma-Aldrich Cat#L2880 14C-Succinic acid ViTrax Inc Cat#VC 195 3H-Citric acid ViTrax Inc Cat#VT 295 SLC13A5 inhibitor BI01383298 TOCRIS Bioscience CAS#2227549-00-8 TRIzol Thermo Fisher Scientific Cat#15596018 SYBR Green master mix Thermo Fisher Scientific Cat#4368708 Horse serum Vector Cat#S-2000 (Continued on next page) e1 Cell Reports 36, 109521, August 10, 2021

    Labeling:

    Article Title: Microglia regulate cortical remyelination via TNFR1-dependent phenotypic polarization.
    Article Snippet: Slides were incubated overnight with the primary antibody at 4 C. Primary antibodies for human multiplex immunofluorescent labeling included caspase1 (1:1500, Proteintech #22915-1-AP), Iba1 (1:5000,Wako #019–19741), IL-18 (1:1000 Abcam #ab191152), IL-1b (1:500, Santa Cruz Biotechnology #sc1250).

    Article Title: Microglia regulate cortical remyelination via TNFR1-dependent phenotypic polarization.
    Article Snippet: Primary antibodies for human immunohistopathology included caspase1 (1:1000, Proteintech #22915-1-AP), Iba1 (1:3000, FUJIFILM Wako #019–19741), IL-18 (1:150, Abcam #ab191152), IL-1b (1:100, Santa Cruz Biotechnology #sc1250).

    Article Title: Antiallergic effect of gami-hyunggyeyeongyotang on ovalbumin-induced allergic rhinitis in mouse and human mast cells.
    Article Snippet: The tissue was then washed three times, and treated with primary antibody overnight at 4 C. Caspase-1 and IL-1b (Santa Cruz Biotechnology) were used as the primary antibodies in a solution with a mixture of bovine serum albumin (GenDepot, Katy, TX, USA) and Triton X100 with PBS.

    Article Title: IDO-Expressing Fibroblasts Protect Islet Beta Cells From Immunological Attack and Reverse Hyperglycemia in Non-Obese Diabetic Mice.
    Article Snippet: Indoleamine 2,3-dioxygenase (IDO) induces immunological tolerance in physiological and pathological conditions.. Therefore, we used dermal fibroblasts with stable IDO expression as a cell therapy to: (i) Investigate the factors determining the efficacy of this cell therapy for autoimmune diabetes in non-obese diabetic (NOD) mice; (ii) Scrutinize the potential immunological mechanisms.. Newly diabetic NOD mice were randomly injected with either 10 10 (10M) or 15 10 (15M) IDO-expressing dermal fibroblasts.

    Article Title: The decoy oligodeoxynucleotide against HIF-1α and STAT5 ameliorates atopic dermatitis-like mouse model
    Article Snippet: The primary antibodies used in this study were as follows: anti-Bax, Bcl-2, COX-2, HIF-1a, IL-4, IL-1b, iNOS, VEGF (Santa Cruz Biotechnology), anti-TNF-a, tryptase, TSLP, p-STAT5, t-STAT5 (Abcam), anti-Bcl-XL, cleaved caspase-3, cyclin D3, cytochrome c, GAPDH (Cell Signaling, Beverly, MA), and anti-filaggrin (Enzo Life Sciences).

    Article Title: Targeting the NLRP3 Inflammasome With Inhibitor MCC950 Prevents Aortic Aneurysms and Dissections in Mice
    Article Snippet: The membranes were blocked for 1 hour in blocking solution comprising Tris-buffered saline containing 5% nonfat dried milk and 0.5% Tween 20 and were incubated overnight with primary antibodies against the following proteins: NLRP3 (AdipoGen Life Sciences), caspase-1 (Santa Cruz Biotechnology), IL-1b (Santa Cruz), and MMP-9 (Santa Cruz).

    Article Title: Mogrol attenuates lipopolysaccharide (LPS)-induced memory impairment and neuroinflammatory responses in mice.
    Article Snippet: This study aimed to evaluate whether mogrol, a main bioactive ingredient of Siraitia grosvenorii, could attenuate LPS-induced memory impairment in mice.. The behavioral tests and immunohistochemical analysis and Western blot were performed.. The present results showed that oral administration of mogrol (20, 40, 80mg/kg) significantly improved LPS-induced memory impairment in mice.

    Article Title: A transepithelial pathway delivers succinate to macrophages, thus perpetuating their pro-inflammatory metabolic state.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIERR Antibodies SUCNR1/GPR91 Novusbio Cat#NBP1-00861 IL-1b Santa Cruz Biotechnology Cat#H-153 donkey anti-goat IgG-HRP Santa Cruz Biotechnology Cat#Sc-2020 CD206 R&D Systems Cat#AF2535 Goat anti-Rabbit IgG (H+L) CrossAdsorbed Secondary Antibody, HRP Thermo Fisher Cat#G21234 b-Actin Sigma-Aldrich Cat#A3854 F4/80 Biogems Cat#02922-80; Clone BM8.1 CD16/CD32 Biogems Cat#08212; Clone 2.4G2 CD11b Biolegend Cat#101215; Clone M1/70 CD80 Biolegend Cat#104725; Clone 16-10A1 SLC26A6 Santa Cruz Cat#sc-515230 SUCNR1 Abbexa Cat#abx318542 Goat anti-Mouse IgG (H+L) CrossAdsorbed Secondary Antibody, Alexa Fluor 488 Invitrogen Cat#A-11001 Goat Anti-Rabbit IgG Antibody (H+L), Biotinylated, R.T.U VECTOR laboratories Cat#BP-9100-50 Biological samples Human fecal samples, serum, gut tissues Severance Hospital N/A Chemicals, peptides, and recombinant proteins cOmplete, EDTA-free Protease Inhibitor Cocktail Roche Cat#11873580001 Pierce Lane Marker Reducing Sample Buffer Thermo Fisher Scientific Cat#39000 Citric acid Sigma-Aldrich Cat#C0759 N-METHYL-D-GLUCAMINE Sigma-Aldrich Cat#M2004 Sodium succinate Sigma-Aldrich Cat#14160 Succinic acid Sigma-Aldrich Cat#S3674 Dextran sulfate sodium (DSS) MP Biomedicals Cat#160110 Chlorophenylalanine Sigma-Aldrich Cat#C6506 2,4,6-trinitrobenzene sulfonic acid (TNBS) Thermo Fisher Scientific Cat#28997 Murine M-CSF Peprotech Cat#315-02 IL-13 Peprotech Cat#210-13 IL-4 Peprotech Cat#214-14 IFNg Peprotech Cat#315-05 thioglycolate Hylabs Cat#TT137 70-mm strainer FLACON Cat#FAL352350 LPS Sigma-Aldrich Cat#L2880 14C-Succinic acid ViTrax Inc Cat#VC 195 3H-Citric acid ViTrax Inc Cat#VT 295 SLC13A5 inhibitor BI01383298 TOCRIS Bioscience CAS#2227549-00-8 TRIzol Thermo Fisher Scientific Cat#15596018 SYBR Green master mix Thermo Fisher Scientific Cat#4368708 Horse serum Vector Cat#S-2000 (Continued on next page) e1 Cell Reports 36, 109521, August 10, 2021

    Recombinant:

    Article Title: Microglia regulate cortical remyelination via TNFR1-dependent phenotypic polarization.
    Article Snippet: Slides were incubated overnight with the primary antibody at 4 C. Primary antibodies for human multiplex immunofluorescent labeling included caspase1 (1:1500, Proteintech #22915-1-AP), Iba1 (1:5000,Wako #019–19741), IL-18 (1:1000 Abcam #ab191152), IL-1b (1:500, Santa Cruz Biotechnology #sc1250).

    Article Title: Microglia regulate cortical remyelination via TNFR1-dependent phenotypic polarization.
    Article Snippet: Primary antibodies for human immunohistopathology included caspase1 (1:1000, Proteintech #22915-1-AP), Iba1 (1:3000, FUJIFILM Wako #019–19741), IL-18 (1:150, Abcam #ab191152), IL-1b (1:100, Santa Cruz Biotechnology #sc1250).

    Article Title: Antiallergic effect of gami-hyunggyeyeongyotang on ovalbumin-induced allergic rhinitis in mouse and human mast cells.
    Article Snippet: The tissue was then washed three times, and treated with primary antibody overnight at 4 C. Caspase-1 and IL-1b (Santa Cruz Biotechnology) were used as the primary antibodies in a solution with a mixture of bovine serum albumin (GenDepot, Katy, TX, USA) and Triton X100 with PBS.

    Article Title: IDO-Expressing Fibroblasts Protect Islet Beta Cells From Immunological Attack and Reverse Hyperglycemia in Non-Obese Diabetic Mice.
    Article Snippet: Indoleamine 2,3-dioxygenase (IDO) induces immunological tolerance in physiological and pathological conditions.. Therefore, we used dermal fibroblasts with stable IDO expression as a cell therapy to: (i) Investigate the factors determining the efficacy of this cell therapy for autoimmune diabetes in non-obese diabetic (NOD) mice; (ii) Scrutinize the potential immunological mechanisms.. Newly diabetic NOD mice were randomly injected with either 10 10 (10M) or 15 10 (15M) IDO-expressing dermal fibroblasts.

    Article Title: The decoy oligodeoxynucleotide against HIF-1α and STAT5 ameliorates atopic dermatitis-like mouse model
    Article Snippet: The primary antibodies used in this study were as follows: anti-Bax, Bcl-2, COX-2, HIF-1a, IL-4, IL-1b, iNOS, VEGF (Santa Cruz Biotechnology), anti-TNF-a, tryptase, TSLP, p-STAT5, t-STAT5 (Abcam), anti-Bcl-XL, cleaved caspase-3, cyclin D3, cytochrome c, GAPDH (Cell Signaling, Beverly, MA), and anti-filaggrin (Enzo Life Sciences).

    Article Title: Targeting the NLRP3 Inflammasome With Inhibitor MCC950 Prevents Aortic Aneurysms and Dissections in Mice
    Article Snippet: The membranes were blocked for 1 hour in blocking solution comprising Tris-buffered saline containing 5% nonfat dried milk and 0.5% Tween 20 and were incubated overnight with primary antibodies against the following proteins: NLRP3 (AdipoGen Life Sciences), caspase-1 (Santa Cruz Biotechnology), IL-1b (Santa Cruz), and MMP-9 (Santa Cruz).

    Article Title: Mogrol attenuates lipopolysaccharide (LPS)-induced memory impairment and neuroinflammatory responses in mice.
    Article Snippet: This study aimed to evaluate whether mogrol, a main bioactive ingredient of Siraitia grosvenorii, could attenuate LPS-induced memory impairment in mice.. The behavioral tests and immunohistochemical analysis and Western blot were performed.. The present results showed that oral administration of mogrol (20, 40, 80mg/kg) significantly improved LPS-induced memory impairment in mice.

    Article Title: A transepithelial pathway delivers succinate to macrophages, thus perpetuating their pro-inflammatory metabolic state.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIERR Antibodies SUCNR1/GPR91 Novusbio Cat#NBP1-00861 IL-1b Santa Cruz Biotechnology Cat#H-153 donkey anti-goat IgG-HRP Santa Cruz Biotechnology Cat#Sc-2020 CD206 R&D Systems Cat#AF2535 Goat anti-Rabbit IgG (H+L) CrossAdsorbed Secondary Antibody, HRP Thermo Fisher Cat#G21234 b-Actin Sigma-Aldrich Cat#A3854 F4/80 Biogems Cat#02922-80; Clone BM8.1 CD16/CD32 Biogems Cat#08212; Clone 2.4G2 CD11b Biolegend Cat#101215; Clone M1/70 CD80 Biolegend Cat#104725; Clone 16-10A1 SLC26A6 Santa Cruz Cat#sc-515230 SUCNR1 Abbexa Cat#abx318542 Goat anti-Mouse IgG (H+L) CrossAdsorbed Secondary Antibody, Alexa Fluor 488 Invitrogen Cat#A-11001 Goat Anti-Rabbit IgG Antibody (H+L), Biotinylated, R.T.U VECTOR laboratories Cat#BP-9100-50 Biological samples Human fecal samples, serum, gut tissues Severance Hospital N/A Chemicals, peptides, and recombinant proteins cOmplete, EDTA-free Protease Inhibitor Cocktail Roche Cat#11873580001 Pierce Lane Marker Reducing Sample Buffer Thermo Fisher Scientific Cat#39000 Citric acid Sigma-Aldrich Cat#C0759 N-METHYL-D-GLUCAMINE Sigma-Aldrich Cat#M2004 Sodium succinate Sigma-Aldrich Cat#14160 Succinic acid Sigma-Aldrich Cat#S3674 Dextran sulfate sodium (DSS) MP Biomedicals Cat#160110 Chlorophenylalanine Sigma-Aldrich Cat#C6506 2,4,6-trinitrobenzene sulfonic acid (TNBS) Thermo Fisher Scientific Cat#28997 Murine M-CSF Peprotech Cat#315-02 IL-13 Peprotech Cat#210-13 IL-4 Peprotech Cat#214-14 IFNg Peprotech Cat#315-05 thioglycolate Hylabs Cat#TT137 70-mm strainer FLACON Cat#FAL352350 LPS Sigma-Aldrich Cat#L2880 14C-Succinic acid ViTrax Inc Cat#VC 195 3H-Citric acid ViTrax Inc Cat#VT 295 SLC13A5 inhibitor BI01383298 TOCRIS Bioscience CAS#2227549-00-8 TRIzol Thermo Fisher Scientific Cat#15596018 SYBR Green master mix Thermo Fisher Scientific Cat#4368708 Horse serum Vector Cat#S-2000 (Continued on next page) e1 Cell Reports 36, 109521, August 10, 2021

    Protease Inhibitor:

    Article Title: Microglia regulate cortical remyelination via TNFR1-dependent phenotypic polarization.
    Article Snippet: Slides were incubated overnight with the primary antibody at 4 C. Primary antibodies for human multiplex immunofluorescent labeling included caspase1 (1:1500, Proteintech #22915-1-AP), Iba1 (1:5000,Wako #019–19741), IL-18 (1:1000 Abcam #ab191152), IL-1b (1:500, Santa Cruz Biotechnology #sc1250).

    Article Title: Microglia regulate cortical remyelination via TNFR1-dependent phenotypic polarization.
    Article Snippet: Primary antibodies for human immunohistopathology included caspase1 (1:1000, Proteintech #22915-1-AP), Iba1 (1:3000, FUJIFILM Wako #019–19741), IL-18 (1:150, Abcam #ab191152), IL-1b (1:100, Santa Cruz Biotechnology #sc1250).

    Article Title: Antiallergic effect of gami-hyunggyeyeongyotang on ovalbumin-induced allergic rhinitis in mouse and human mast cells.
    Article Snippet: The tissue was then washed three times, and treated with primary antibody overnight at 4 C. Caspase-1 and IL-1b (Santa Cruz Biotechnology) were used as the primary antibodies in a solution with a mixture of bovine serum albumin (GenDepot, Katy, TX, USA) and Triton X100 with PBS.

    Article Title: IDO-Expressing Fibroblasts Protect Islet Beta Cells From Immunological Attack and Reverse Hyperglycemia in Non-Obese Diabetic Mice.
    Article Snippet: Indoleamine 2,3-dioxygenase (IDO) induces immunological tolerance in physiological and pathological conditions.. Therefore, we used dermal fibroblasts with stable IDO expression as a cell therapy to: (i) Investigate the factors determining the efficacy of this cell therapy for autoimmune diabetes in non-obese diabetic (NOD) mice; (ii) Scrutinize the potential immunological mechanisms.. Newly diabetic NOD mice were randomly injected with either 10 10 (10M) or 15 10 (15M) IDO-expressing dermal fibroblasts.

    Article Title: The decoy oligodeoxynucleotide against HIF-1α and STAT5 ameliorates atopic dermatitis-like mouse model
    Article Snippet: The primary antibodies used in this study were as follows: anti-Bax, Bcl-2, COX-2, HIF-1a, IL-4, IL-1b, iNOS, VEGF (Santa Cruz Biotechnology), anti-TNF-a, tryptase, TSLP, p-STAT5, t-STAT5 (Abcam), anti-Bcl-XL, cleaved caspase-3, cyclin D3, cytochrome c, GAPDH (Cell Signaling, Beverly, MA), and anti-filaggrin (Enzo Life Sciences).

    Article Title: Targeting the NLRP3 Inflammasome With Inhibitor MCC950 Prevents Aortic Aneurysms and Dissections in Mice
    Article Snippet: The membranes were blocked for 1 hour in blocking solution comprising Tris-buffered saline containing 5% nonfat dried milk and 0.5% Tween 20 and were incubated overnight with primary antibodies against the following proteins: NLRP3 (AdipoGen Life Sciences), caspase-1 (Santa Cruz Biotechnology), IL-1b (Santa Cruz), and MMP-9 (Santa Cruz).

    Article Title: Mogrol attenuates lipopolysaccharide (LPS)-induced memory impairment and neuroinflammatory responses in mice.
    Article Snippet: This study aimed to evaluate whether mogrol, a main bioactive ingredient of Siraitia grosvenorii, could attenuate LPS-induced memory impairment in mice.. The behavioral tests and immunohistochemical analysis and Western blot were performed.. The present results showed that oral administration of mogrol (20, 40, 80mg/kg) significantly improved LPS-induced memory impairment in mice.

    Article Title: A transepithelial pathway delivers succinate to macrophages, thus perpetuating their pro-inflammatory metabolic state.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIERR Antibodies SUCNR1/GPR91 Novusbio Cat#NBP1-00861 IL-1b Santa Cruz Biotechnology Cat#H-153 donkey anti-goat IgG-HRP Santa Cruz Biotechnology Cat#Sc-2020 CD206 R&D Systems Cat#AF2535 Goat anti-Rabbit IgG (H+L) CrossAdsorbed Secondary Antibody, HRP Thermo Fisher Cat#G21234 b-Actin Sigma-Aldrich Cat#A3854 F4/80 Biogems Cat#02922-80; Clone BM8.1 CD16/CD32 Biogems Cat#08212; Clone 2.4G2 CD11b Biolegend Cat#101215; Clone M1/70 CD80 Biolegend Cat#104725; Clone 16-10A1 SLC26A6 Santa Cruz Cat#sc-515230 SUCNR1 Abbexa Cat#abx318542 Goat anti-Mouse IgG (H+L) CrossAdsorbed Secondary Antibody, Alexa Fluor 488 Invitrogen Cat#A-11001 Goat Anti-Rabbit IgG Antibody (H+L), Biotinylated, R.T.U VECTOR laboratories Cat#BP-9100-50 Biological samples Human fecal samples, serum, gut tissues Severance Hospital N/A Chemicals, peptides, and recombinant proteins cOmplete, EDTA-free Protease Inhibitor Cocktail Roche Cat#11873580001 Pierce Lane Marker Reducing Sample Buffer Thermo Fisher Scientific Cat#39000 Citric acid Sigma-Aldrich Cat#C0759 N-METHYL-D-GLUCAMINE Sigma-Aldrich Cat#M2004 Sodium succinate Sigma-Aldrich Cat#14160 Succinic acid Sigma-Aldrich Cat#S3674 Dextran sulfate sodium (DSS) MP Biomedicals Cat#160110 Chlorophenylalanine Sigma-Aldrich Cat#C6506 2,4,6-trinitrobenzene sulfonic acid (TNBS) Thermo Fisher Scientific Cat#28997 Murine M-CSF Peprotech Cat#315-02 IL-13 Peprotech Cat#210-13 IL-4 Peprotech Cat#214-14 IFNg Peprotech Cat#315-05 thioglycolate Hylabs Cat#TT137 70-mm strainer FLACON Cat#FAL352350 LPS Sigma-Aldrich Cat#L2880 14C-Succinic acid ViTrax Inc Cat#VC 195 3H-Citric acid ViTrax Inc Cat#VT 295 SLC13A5 inhibitor BI01383298 TOCRIS Bioscience CAS#2227549-00-8 TRIzol Thermo Fisher Scientific Cat#15596018 SYBR Green master mix Thermo Fisher Scientific Cat#4368708 Horse serum Vector Cat#S-2000 (Continued on next page) e1 Cell Reports 36, 109521, August 10, 2021

    Marker:

    Article Title: Microglia regulate cortical remyelination via TNFR1-dependent phenotypic polarization.
    Article Snippet: Slides were incubated overnight with the primary antibody at 4 C. Primary antibodies for human multiplex immunofluorescent labeling included caspase1 (1:1500, Proteintech #22915-1-AP), Iba1 (1:5000,Wako #019–19741), IL-18 (1:1000 Abcam #ab191152), IL-1b (1:500, Santa Cruz Biotechnology #sc1250).

    Article Title: Microglia regulate cortical remyelination via TNFR1-dependent phenotypic polarization.
    Article Snippet: Primary antibodies for human immunohistopathology included caspase1 (1:1000, Proteintech #22915-1-AP), Iba1 (1:3000, FUJIFILM Wako #019–19741), IL-18 (1:150, Abcam #ab191152), IL-1b (1:100, Santa Cruz Biotechnology #sc1250).

    Article Title: Antiallergic effect of gami-hyunggyeyeongyotang on ovalbumin-induced allergic rhinitis in mouse and human mast cells.
    Article Snippet: The tissue was then washed three times, and treated with primary antibody overnight at 4 C. Caspase-1 and IL-1b (Santa Cruz Biotechnology) were used as the primary antibodies in a solution with a mixture of bovine serum albumin (GenDepot, Katy, TX, USA) and Triton X100 with PBS.

    Article Title: IDO-Expressing Fibroblasts Protect Islet Beta Cells From Immunological Attack and Reverse Hyperglycemia in Non-Obese Diabetic Mice.
    Article Snippet: Indoleamine 2,3-dioxygenase (IDO) induces immunological tolerance in physiological and pathological conditions.. Therefore, we used dermal fibroblasts with stable IDO expression as a cell therapy to: (i) Investigate the factors determining the efficacy of this cell therapy for autoimmune diabetes in non-obese diabetic (NOD) mice; (ii) Scrutinize the potential immunological mechanisms.. Newly diabetic NOD mice were randomly injected with either 10 10 (10M) or 15 10 (15M) IDO-expressing dermal fibroblasts.

    Article Title: The decoy oligodeoxynucleotide against HIF-1α and STAT5 ameliorates atopic dermatitis-like mouse model
    Article Snippet: The primary antibodies used in this study were as follows: anti-Bax, Bcl-2, COX-2, HIF-1a, IL-4, IL-1b, iNOS, VEGF (Santa Cruz Biotechnology), anti-TNF-a, tryptase, TSLP, p-STAT5, t-STAT5 (Abcam), anti-Bcl-XL, cleaved caspase-3, cyclin D3, cytochrome c, GAPDH (Cell Signaling, Beverly, MA), and anti-filaggrin (Enzo Life Sciences).

    Article Title: Targeting the NLRP3 Inflammasome With Inhibitor MCC950 Prevents Aortic Aneurysms and Dissections in Mice
    Article Snippet: The membranes were blocked for 1 hour in blocking solution comprising Tris-buffered saline containing 5% nonfat dried milk and 0.5% Tween 20 and were incubated overnight with primary antibodies against the following proteins: NLRP3 (AdipoGen Life Sciences), caspase-1 (Santa Cruz Biotechnology), IL-1b (Santa Cruz), and MMP-9 (Santa Cruz).

    Article Title: Mogrol attenuates lipopolysaccharide (LPS)-induced memory impairment and neuroinflammatory responses in mice.
    Article Snippet: This study aimed to evaluate whether mogrol, a main bioactive ingredient of Siraitia grosvenorii, could attenuate LPS-induced memory impairment in mice.. The behavioral tests and immunohistochemical analysis and Western blot were performed.. The present results showed that oral administration of mogrol (20, 40, 80mg/kg) significantly improved LPS-induced memory impairment in mice.

    Article Title: A transepithelial pathway delivers succinate to macrophages, thus perpetuating their pro-inflammatory metabolic state.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIERR Antibodies SUCNR1/GPR91 Novusbio Cat#NBP1-00861 IL-1b Santa Cruz Biotechnology Cat#H-153 donkey anti-goat IgG-HRP Santa Cruz Biotechnology Cat#Sc-2020 CD206 R&D Systems Cat#AF2535 Goat anti-Rabbit IgG (H+L) CrossAdsorbed Secondary Antibody, HRP Thermo Fisher Cat#G21234 b-Actin Sigma-Aldrich Cat#A3854 F4/80 Biogems Cat#02922-80; Clone BM8.1 CD16/CD32 Biogems Cat#08212; Clone 2.4G2 CD11b Biolegend Cat#101215; Clone M1/70 CD80 Biolegend Cat#104725; Clone 16-10A1 SLC26A6 Santa Cruz Cat#sc-515230 SUCNR1 Abbexa Cat#abx318542 Goat anti-Mouse IgG (H+L) CrossAdsorbed Secondary Antibody, Alexa Fluor 488 Invitrogen Cat#A-11001 Goat Anti-Rabbit IgG Antibody (H+L), Biotinylated, R.T.U VECTOR laboratories Cat#BP-9100-50 Biological samples Human fecal samples, serum, gut tissues Severance Hospital N/A Chemicals, peptides, and recombinant proteins cOmplete, EDTA-free Protease Inhibitor Cocktail Roche Cat#11873580001 Pierce Lane Marker Reducing Sample Buffer Thermo Fisher Scientific Cat#39000 Citric acid Sigma-Aldrich Cat#C0759 N-METHYL-D-GLUCAMINE Sigma-Aldrich Cat#M2004 Sodium succinate Sigma-Aldrich Cat#14160 Succinic acid Sigma-Aldrich Cat#S3674 Dextran sulfate sodium (DSS) MP Biomedicals Cat#160110 Chlorophenylalanine Sigma-Aldrich Cat#C6506 2,4,6-trinitrobenzene sulfonic acid (TNBS) Thermo Fisher Scientific Cat#28997 Murine M-CSF Peprotech Cat#315-02 IL-13 Peprotech Cat#210-13 IL-4 Peprotech Cat#214-14 IFNg Peprotech Cat#315-05 thioglycolate Hylabs Cat#TT137 70-mm strainer FLACON Cat#FAL352350 LPS Sigma-Aldrich Cat#L2880 14C-Succinic acid ViTrax Inc Cat#VC 195 3H-Citric acid ViTrax Inc Cat#VT 295 SLC13A5 inhibitor BI01383298 TOCRIS Bioscience CAS#2227549-00-8 TRIzol Thermo Fisher Scientific Cat#15596018 SYBR Green master mix Thermo Fisher Scientific Cat#4368708 Horse serum Vector Cat#S-2000 (Continued on next page) e1 Cell Reports 36, 109521, August 10, 2021

    SYBR Green Assay:

    Article Title: Microglia regulate cortical remyelination via TNFR1-dependent phenotypic polarization.
    Article Snippet: Slides were incubated overnight with the primary antibody at 4 C. Primary antibodies for human multiplex immunofluorescent labeling included caspase1 (1:1500, Proteintech #22915-1-AP), Iba1 (1:5000,Wako #019–19741), IL-18 (1:1000 Abcam #ab191152), IL-1b (1:500, Santa Cruz Biotechnology #sc1250).

    Article Title: Microglia regulate cortical remyelination via TNFR1-dependent phenotypic polarization.
    Article Snippet: Primary antibodies for human immunohistopathology included caspase1 (1:1000, Proteintech #22915-1-AP), Iba1 (1:3000, FUJIFILM Wako #019–19741), IL-18 (1:150, Abcam #ab191152), IL-1b (1:100, Santa Cruz Biotechnology #sc1250).

    Article Title: Antiallergic effect of gami-hyunggyeyeongyotang on ovalbumin-induced allergic rhinitis in mouse and human mast cells.
    Article Snippet: The tissue was then washed three times, and treated with primary antibody overnight at 4 C. Caspase-1 and IL-1b (Santa Cruz Biotechnology) were used as the primary antibodies in a solution with a mixture of bovine serum albumin (GenDepot, Katy, TX, USA) and Triton X100 with PBS.

    Article Title: IDO-Expressing Fibroblasts Protect Islet Beta Cells From Immunological Attack and Reverse Hyperglycemia in Non-Obese Diabetic Mice.
    Article Snippet: Indoleamine 2,3-dioxygenase (IDO) induces immunological tolerance in physiological and pathological conditions.. Therefore, we used dermal fibroblasts with stable IDO expression as a cell therapy to: (i) Investigate the factors determining the efficacy of this cell therapy for autoimmune diabetes in non-obese diabetic (NOD) mice; (ii) Scrutinize the potential immunological mechanisms.. Newly diabetic NOD mice were randomly injected with either 10 10 (10M) or 15 10 (15M) IDO-expressing dermal fibroblasts.

    Article Title: The decoy oligodeoxynucleotide against HIF-1α and STAT5 ameliorates atopic dermatitis-like mouse model
    Article Snippet: The primary antibodies used in this study were as follows: anti-Bax, Bcl-2, COX-2, HIF-1a, IL-4, IL-1b, iNOS, VEGF (Santa Cruz Biotechnology), anti-TNF-a, tryptase, TSLP, p-STAT5, t-STAT5 (Abcam), anti-Bcl-XL, cleaved caspase-3, cyclin D3, cytochrome c, GAPDH (Cell Signaling, Beverly, MA), and anti-filaggrin (Enzo Life Sciences).

    Article Title: Targeting the NLRP3 Inflammasome With Inhibitor MCC950 Prevents Aortic Aneurysms and Dissections in Mice
    Article Snippet: The membranes were blocked for 1 hour in blocking solution comprising Tris-buffered saline containing 5% nonfat dried milk and 0.5% Tween 20 and were incubated overnight with primary antibodies against the following proteins: NLRP3 (AdipoGen Life Sciences), caspase-1 (Santa Cruz Biotechnology), IL-1b (Santa Cruz), and MMP-9 (Santa Cruz).

    Article Title: Mogrol attenuates lipopolysaccharide (LPS)-induced memory impairment and neuroinflammatory responses in mice.
    Article Snippet: This study aimed to evaluate whether mogrol, a main bioactive ingredient of Siraitia grosvenorii, could attenuate LPS-induced memory impairment in mice.. The behavioral tests and immunohistochemical analysis and Western blot were performed.. The present results showed that oral administration of mogrol (20, 40, 80mg/kg) significantly improved LPS-induced memory impairment in mice.

    Article Title: A transepithelial pathway delivers succinate to macrophages, thus perpetuating their pro-inflammatory metabolic state.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIERR Antibodies SUCNR1/GPR91 Novusbio Cat#NBP1-00861 IL-1b Santa Cruz Biotechnology Cat#H-153 donkey anti-goat IgG-HRP Santa Cruz Biotechnology Cat#Sc-2020 CD206 R&D Systems Cat#AF2535 Goat anti-Rabbit IgG (H+L) CrossAdsorbed Secondary Antibody, HRP Thermo Fisher Cat#G21234 b-Actin Sigma-Aldrich Cat#A3854 F4/80 Biogems Cat#02922-80; Clone BM8.1 CD16/CD32 Biogems Cat#08212; Clone 2.4G2 CD11b Biolegend Cat#101215; Clone M1/70 CD80 Biolegend Cat#104725; Clone 16-10A1 SLC26A6 Santa Cruz Cat#sc-515230 SUCNR1 Abbexa Cat#abx318542 Goat anti-Mouse IgG (H+L) CrossAdsorbed Secondary Antibody, Alexa Fluor 488 Invitrogen Cat#A-11001 Goat Anti-Rabbit IgG Antibody (H+L), Biotinylated, R.T.U VECTOR laboratories Cat#BP-9100-50 Biological samples Human fecal samples, serum, gut tissues Severance Hospital N/A Chemicals, peptides, and recombinant proteins cOmplete, EDTA-free Protease Inhibitor Cocktail Roche Cat#11873580001 Pierce Lane Marker Reducing Sample Buffer Thermo Fisher Scientific Cat#39000 Citric acid Sigma-Aldrich Cat#C0759 N-METHYL-D-GLUCAMINE Sigma-Aldrich Cat#M2004 Sodium succinate Sigma-Aldrich Cat#14160 Succinic acid Sigma-Aldrich Cat#S3674 Dextran sulfate sodium (DSS) MP Biomedicals Cat#160110 Chlorophenylalanine Sigma-Aldrich Cat#C6506 2,4,6-trinitrobenzene sulfonic acid (TNBS) Thermo Fisher Scientific Cat#28997 Murine M-CSF Peprotech Cat#315-02 IL-13 Peprotech Cat#210-13 IL-4 Peprotech Cat#214-14 IFNg Peprotech Cat#315-05 thioglycolate Hylabs Cat#TT137 70-mm strainer FLACON Cat#FAL352350 LPS Sigma-Aldrich Cat#L2880 14C-Succinic acid ViTrax Inc Cat#VC 195 3H-Citric acid ViTrax Inc Cat#VT 295 SLC13A5 inhibitor BI01383298 TOCRIS Bioscience CAS#2227549-00-8 TRIzol Thermo Fisher Scientific Cat#15596018 SYBR Green master mix Thermo Fisher Scientific Cat#4368708 Horse serum Vector Cat#S-2000 (Continued on next page) e1 Cell Reports 36, 109521, August 10, 2021

    Blocking Assay:

    Article Title: Microglia regulate cortical remyelination via TNFR1-dependent phenotypic polarization.
    Article Snippet: Slides were incubated overnight with the primary antibody at 4 C. Primary antibodies for human multiplex immunofluorescent labeling included caspase1 (1:1500, Proteintech #22915-1-AP), Iba1 (1:5000,Wako #019–19741), IL-18 (1:1000 Abcam #ab191152), IL-1b (1:500, Santa Cruz Biotechnology #sc1250).

    Article Title: Microglia regulate cortical remyelination via TNFR1-dependent phenotypic polarization.
    Article Snippet: Primary antibodies for human immunohistopathology included caspase1 (1:1000, Proteintech #22915-1-AP), Iba1 (1:3000, FUJIFILM Wako #019–19741), IL-18 (1:150, Abcam #ab191152), IL-1b (1:100, Santa Cruz Biotechnology #sc1250).

    Article Title: Antiallergic effect of gami-hyunggyeyeongyotang on ovalbumin-induced allergic rhinitis in mouse and human mast cells.
    Article Snippet: The tissue was then washed three times, and treated with primary antibody overnight at 4 C. Caspase-1 and IL-1b (Santa Cruz Biotechnology) were used as the primary antibodies in a solution with a mixture of bovine serum albumin (GenDepot, Katy, TX, USA) and Triton X100 with PBS.

    Article Title: IDO-Expressing Fibroblasts Protect Islet Beta Cells From Immunological Attack and Reverse Hyperglycemia in Non-Obese Diabetic Mice.
    Article Snippet: Indoleamine 2,3-dioxygenase (IDO) induces immunological tolerance in physiological and pathological conditions.. Therefore, we used dermal fibroblasts with stable IDO expression as a cell therapy to: (i) Investigate the factors determining the efficacy of this cell therapy for autoimmune diabetes in non-obese diabetic (NOD) mice; (ii) Scrutinize the potential immunological mechanisms.. Newly diabetic NOD mice were randomly injected with either 10 10 (10M) or 15 10 (15M) IDO-expressing dermal fibroblasts.

    Article Title: The decoy oligodeoxynucleotide against HIF-1α and STAT5 ameliorates atopic dermatitis-like mouse model
    Article Snippet: The primary antibodies used in this study were as follows: anti-Bax, Bcl-2, COX-2, HIF-1a, IL-4, IL-1b, iNOS, VEGF (Santa Cruz Biotechnology), anti-TNF-a, tryptase, TSLP, p-STAT5, t-STAT5 (Abcam), anti-Bcl-XL, cleaved caspase-3, cyclin D3, cytochrome c, GAPDH (Cell Signaling, Beverly, MA), and anti-filaggrin (Enzo Life Sciences).

    Article Title: Targeting the NLRP3 Inflammasome With Inhibitor MCC950 Prevents Aortic Aneurysms and Dissections in Mice
    Article Snippet: The membranes were blocked for 1 hour in blocking solution comprising Tris-buffered saline containing 5% nonfat dried milk and 0.5% Tween 20 and were incubated overnight with primary antibodies against the following proteins: NLRP3 (AdipoGen Life Sciences), caspase-1 (Santa Cruz Biotechnology), IL-1b (Santa Cruz), and MMP-9 (Santa Cruz).

    Article Title: Mogrol attenuates lipopolysaccharide (LPS)-induced memory impairment and neuroinflammatory responses in mice.
    Article Snippet: This study aimed to evaluate whether mogrol, a main bioactive ingredient of Siraitia grosvenorii, could attenuate LPS-induced memory impairment in mice.. The behavioral tests and immunohistochemical analysis and Western blot were performed.. The present results showed that oral administration of mogrol (20, 40, 80mg/kg) significantly improved LPS-induced memory impairment in mice.

    Article Title: A transepithelial pathway delivers succinate to macrophages, thus perpetuating their pro-inflammatory metabolic state.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIERR Antibodies SUCNR1/GPR91 Novusbio Cat#NBP1-00861 IL-1b Santa Cruz Biotechnology Cat#H-153 donkey anti-goat IgG-HRP Santa Cruz Biotechnology Cat#Sc-2020 CD206 R&D Systems Cat#AF2535 Goat anti-Rabbit IgG (H+L) CrossAdsorbed Secondary Antibody, HRP Thermo Fisher Cat#G21234 b-Actin Sigma-Aldrich Cat#A3854 F4/80 Biogems Cat#02922-80; Clone BM8.1 CD16/CD32 Biogems Cat#08212; Clone 2.4G2 CD11b Biolegend Cat#101215; Clone M1/70 CD80 Biolegend Cat#104725; Clone 16-10A1 SLC26A6 Santa Cruz Cat#sc-515230 SUCNR1 Abbexa Cat#abx318542 Goat anti-Mouse IgG (H+L) CrossAdsorbed Secondary Antibody, Alexa Fluor 488 Invitrogen Cat#A-11001 Goat Anti-Rabbit IgG Antibody (H+L), Biotinylated, R.T.U VECTOR laboratories Cat#BP-9100-50 Biological samples Human fecal samples, serum, gut tissues Severance Hospital N/A Chemicals, peptides, and recombinant proteins cOmplete, EDTA-free Protease Inhibitor Cocktail Roche Cat#11873580001 Pierce Lane Marker Reducing Sample Buffer Thermo Fisher Scientific Cat#39000 Citric acid Sigma-Aldrich Cat#C0759 N-METHYL-D-GLUCAMINE Sigma-Aldrich Cat#M2004 Sodium succinate Sigma-Aldrich Cat#14160 Succinic acid Sigma-Aldrich Cat#S3674 Dextran sulfate sodium (DSS) MP Biomedicals Cat#160110 Chlorophenylalanine Sigma-Aldrich Cat#C6506 2,4,6-trinitrobenzene sulfonic acid (TNBS) Thermo Fisher Scientific Cat#28997 Murine M-CSF Peprotech Cat#315-02 IL-13 Peprotech Cat#210-13 IL-4 Peprotech Cat#214-14 IFNg Peprotech Cat#315-05 thioglycolate Hylabs Cat#TT137 70-mm strainer FLACON Cat#FAL352350 LPS Sigma-Aldrich Cat#L2880 14C-Succinic acid ViTrax Inc Cat#VC 195 3H-Citric acid ViTrax Inc Cat#VT 295 SLC13A5 inhibitor BI01383298 TOCRIS Bioscience CAS#2227549-00-8 TRIzol Thermo Fisher Scientific Cat#15596018 SYBR Green master mix Thermo Fisher Scientific Cat#4368708 Horse serum Vector Cat#S-2000 (Continued on next page) e1 Cell Reports 36, 109521, August 10, 2021

    Saline:

    Article Title: Microglia regulate cortical remyelination via TNFR1-dependent phenotypic polarization.
    Article Snippet: Slides were incubated overnight with the primary antibody at 4 C. Primary antibodies for human multiplex immunofluorescent labeling included caspase1 (1:1500, Proteintech #22915-1-AP), Iba1 (1:5000,Wako #019–19741), IL-18 (1:1000 Abcam #ab191152), IL-1b (1:500, Santa Cruz Biotechnology #sc1250).

    Article Title: Microglia regulate cortical remyelination via TNFR1-dependent phenotypic polarization.
    Article Snippet: Primary antibodies for human immunohistopathology included caspase1 (1:1000, Proteintech #22915-1-AP), Iba1 (1:3000, FUJIFILM Wako #019–19741), IL-18 (1:150, Abcam #ab191152), IL-1b (1:100, Santa Cruz Biotechnology #sc1250).

    Article Title: Antiallergic effect of gami-hyunggyeyeongyotang on ovalbumin-induced allergic rhinitis in mouse and human mast cells.
    Article Snippet: The tissue was then washed three times, and treated with primary antibody overnight at 4 C. Caspase-1 and IL-1b (Santa Cruz Biotechnology) were used as the primary antibodies in a solution with a mixture of bovine serum albumin (GenDepot, Katy, TX, USA) and Triton X100 with PBS.

    Article Title: IDO-Expressing Fibroblasts Protect Islet Beta Cells From Immunological Attack and Reverse Hyperglycemia in Non-Obese Diabetic Mice.
    Article Snippet: Indoleamine 2,3-dioxygenase (IDO) induces immunological tolerance in physiological and pathological conditions.. Therefore, we used dermal fibroblasts with stable IDO expression as a cell therapy to: (i) Investigate the factors determining the efficacy of this cell therapy for autoimmune diabetes in non-obese diabetic (NOD) mice; (ii) Scrutinize the potential immunological mechanisms.. Newly diabetic NOD mice were randomly injected with either 10 10 (10M) or 15 10 (15M) IDO-expressing dermal fibroblasts.

    Article Title: The decoy oligodeoxynucleotide against HIF-1α and STAT5 ameliorates atopic dermatitis-like mouse model
    Article Snippet: The primary antibodies used in this study were as follows: anti-Bax, Bcl-2, COX-2, HIF-1a, IL-4, IL-1b, iNOS, VEGF (Santa Cruz Biotechnology), anti-TNF-a, tryptase, TSLP, p-STAT5, t-STAT5 (Abcam), anti-Bcl-XL, cleaved caspase-3, cyclin D3, cytochrome c, GAPDH (Cell Signaling, Beverly, MA), and anti-filaggrin (Enzo Life Sciences).

    Article Title: Targeting the NLRP3 Inflammasome With Inhibitor MCC950 Prevents Aortic Aneurysms and Dissections in Mice
    Article Snippet: The membranes were blocked for 1 hour in blocking solution comprising Tris-buffered saline containing 5% nonfat dried milk and 0.5% Tween 20 and were incubated overnight with primary antibodies against the following proteins: NLRP3 (AdipoGen Life Sciences), caspase-1 (Santa Cruz Biotechnology), IL-1b (Santa Cruz), and MMP-9 (Santa Cruz).

    Article Title: Mogrol attenuates lipopolysaccharide (LPS)-induced memory impairment and neuroinflammatory responses in mice.
    Article Snippet: This study aimed to evaluate whether mogrol, a main bioactive ingredient of Siraitia grosvenorii, could attenuate LPS-induced memory impairment in mice.. The behavioral tests and immunohistochemical analysis and Western blot were performed.. The present results showed that oral administration of mogrol (20, 40, 80mg/kg) significantly improved LPS-induced memory impairment in mice.

    Article Title: A transepithelial pathway delivers succinate to macrophages, thus perpetuating their pro-inflammatory metabolic state.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIERR Antibodies SUCNR1/GPR91 Novusbio Cat#NBP1-00861 IL-1b Santa Cruz Biotechnology Cat#H-153 donkey anti-goat IgG-HRP Santa Cruz Biotechnology Cat#Sc-2020 CD206 R&D Systems Cat#AF2535 Goat anti-Rabbit IgG (H+L) CrossAdsorbed Secondary Antibody, HRP Thermo Fisher Cat#G21234 b-Actin Sigma-Aldrich Cat#A3854 F4/80 Biogems Cat#02922-80; Clone BM8.1 CD16/CD32 Biogems Cat#08212; Clone 2.4G2 CD11b Biolegend Cat#101215; Clone M1/70 CD80 Biolegend Cat#104725; Clone 16-10A1 SLC26A6 Santa Cruz Cat#sc-515230 SUCNR1 Abbexa Cat#abx318542 Goat anti-Mouse IgG (H+L) CrossAdsorbed Secondary Antibody, Alexa Fluor 488 Invitrogen Cat#A-11001 Goat Anti-Rabbit IgG Antibody (H+L), Biotinylated, R.T.U VECTOR laboratories Cat#BP-9100-50 Biological samples Human fecal samples, serum, gut tissues Severance Hospital N/A Chemicals, peptides, and recombinant proteins cOmplete, EDTA-free Protease Inhibitor Cocktail Roche Cat#11873580001 Pierce Lane Marker Reducing Sample Buffer Thermo Fisher Scientific Cat#39000 Citric acid Sigma-Aldrich Cat#C0759 N-METHYL-D-GLUCAMINE Sigma-Aldrich Cat#M2004 Sodium succinate Sigma-Aldrich Cat#14160 Succinic acid Sigma-Aldrich Cat#S3674 Dextran sulfate sodium (DSS) MP Biomedicals Cat#160110 Chlorophenylalanine Sigma-Aldrich Cat#C6506 2,4,6-trinitrobenzene sulfonic acid (TNBS) Thermo Fisher Scientific Cat#28997 Murine M-CSF Peprotech Cat#315-02 IL-13 Peprotech Cat#210-13 IL-4 Peprotech Cat#214-14 IFNg Peprotech Cat#315-05 thioglycolate Hylabs Cat#TT137 70-mm strainer FLACON Cat#FAL352350 LPS Sigma-Aldrich Cat#L2880 14C-Succinic acid ViTrax Inc Cat#VC 195 3H-Citric acid ViTrax Inc Cat#VT 295 SLC13A5 inhibitor BI01383298 TOCRIS Bioscience CAS#2227549-00-8 TRIzol Thermo Fisher Scientific Cat#15596018 SYBR Green master mix Thermo Fisher Scientific Cat#4368708 Horse serum Vector Cat#S-2000 (Continued on next page) e1 Cell Reports 36, 109521, August 10, 2021



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